Constructing Buildings and Harmonic Maps

In a continuation of our previous work, we outline a theory which should lead to the construction of a universal pre-building and versal building with a $\phi$-harmonic map from a Riemann surface, in the case of two-dimensional buildings for the group $SL_3$. This will provide a generalization of the space of leaves of the foliation defined by a quadratic differential in the classical theory for $SL_2$. Our conjectural construction would determine the exponents for $SL_3$ WKB problems, and it can be put into practice on examples.Comment: 61 pages, 24 figures. Comments are welcom

Cerium oxide nanoparticles for the detection of antimicrobial resistance

The rise of antimicrobial resistance demands the development of more rapid screening methods for the detection of antimicrobial resistance in clinical samples to both give the patient the proper treatment and expedite the treatment of patients. Cerium oxide nanoparticles may serve a useful role in diagnostics due to their ability to exist in a mixed valence state and act as either oxidizing agents or reducing agents. Considering that cerium oxide nanoparticles have been shown to shift in absorbance upon oxidation, a useful method of antimicrobial resistance detection could be based on the oxidation of cerium oxide nanoparticles. Herein, an assay is described whereby cerium oxide nanoparticle oxidation is a function of glucose metabolism of bacterial samples in the presence of an antimicrobial agent. Cerium oxide nanoparticles were shown to have an absorbance in the range of 395nm upon oxidation by hydrogen peroxide whereas mixed valence cerium oxide nanoparticles lacked an absorbance around 395nm. In the presence the hydrogen peroxide-producing glucose oxidase and either increasing concentrations of glucose or bacterial medium supplemented with increasing concentrations of glucose, cerium oxide nanoparticles were shown to increase in absorbance at 395nm. This oxidation assay was capable of measuring differences in the absorbance of E. coli and S. aureus samples grown in the presence of inhibitory and non-inhibitory concentrations of ampicillin in as little as six hours. Therefore, this cerium oxide nanoparticle oxidation assay may be very useful for use in clinical laboratories for the detection of antimicrobial resistance due to the relatively low cost, no requirement for specialized equipment and, most importantly, the reduced incubation time of the assay to as little as six hours compared to current gold standard antimicrobial resistance detection methods that require 24 hours.; This assay may thus also help partially circumvent the issue of knowledge of antimicrobial resistance in infected patients before prescribing improper regimens

Observational studies in group testing and potential applications.

The use of group testing to identify individuals with targeted outcomes in a population can greatly improve the efficiency, speed, and cost effectiveness of testing a population for an outcome, or at least for identifying the prevalence of an outcome in a population. The implementation of causal inference techniques can provide the basis for an observational study that would allow an investigator to gather estimates for treatment effectiveness if group testing was conducted on the population in a certain way. This thesis examines a simulation of the above outlined principles in order to demonstrate a potential application for determining treatment efficacy from observational data obtained via testing for disease outcome in a partially treated population. It is made evident that it is reasonable to make conclusions about treatment conditional incidence of an outcome in a sample of tested individuals based on outcome tests conducted on groups of individuals. Examining group study observations in the manner described in this thesis will allow researchers to estimate treatment effectiveness from partial data in situations where outcome testing may have been limited or where quick results are required from limited data

Благополучие и проблема устойчивого развития общества

В статье анализируется взаимосвязь благополучия и устойчивого развития. Цель статьи - продемонстрировать, что общественное развитие приобретает все большую зависимость от ценностных параметров, формирующих траекторию общественного движения. В качестве базового основания для анализа взаимосвязи рассматриваемых категорий взята модель повседневных практик человека, исходя из которой благополучие выступает механизмом регулирования социально значимых процессов, оказывающих влияние на жизненные ситуации и среды жизнедеятельности человека и общества в целом. Проблема благополучия мировым научным сообществом исследуется в разных аспектах, но в то же время не может быть отнесена только к предмету строго научного и экспериментального исследования, что обусловлено природой благополучия, рассматриваемого как сложный социальный феномен по предназначению и применению. The paper introduces the analysis of the social welfare and sustainability. The aim of the paper is to demonstratethat the social development becomes more dependable on valuable parameters that form the locus of the socialmovement. The model of the socio-practical existence serves as a basis for the analysis of the interaction betweenthe categories under study. The socio-practical existence trends towards the satisfaction of human needs.This aspect is more important in the modern society. The simple use of consumer amenities develops into theinstrument of the social identity construction and the individual sociocultural integration in the society. Welfareis considered as the ontological concept that establishes the measurement of social reality and it is deflected duringthe processes of the individual existence. This allows the determination of the welfare by the universal resourcewhich is able to increase the degree of stability of the social object functioning in a context of uncertainty

Fano hypersurfaces and Calabi-Yau supermanifolds

In this paper, we study the geometrical interpretations associated with Sethi's proposed general correspondence between N = 2 Landau-Ginzburg orbifolds with integral \hat{c} and N = 2 nonlinear sigma models. We focus on the supervarieties associated with \hat{c} = 3 Gepner models. In the process, we test a conjecture regarding the superdimension of the singular locus of these supervarieties. The supervarieties are defined by a hypersurface \widetilde{W} = 0 in a weighted superprojective space and have vanishing super-first Chern class. Here, \widetilde{W} is the modified superpotential obtained by adding as necessary to the Gepner superpotential a boson mass term and/or fermion bilinears so that the superdimension of the supervariety is equal to \hat{c}. When Sethi's proposal calls for adding fermion bilinears, setting the bosonic part of \widetilde{W} (denoted by \widetilde{W}_{bos}) equal to zero defines a Fano hypersurface embedded in a weighted projective space. In this case, if the Newton polytope of \widetilde{W}_{bos} admits a nef partition, then the Landau-Ginzburg orbifold can be given a geometrical interpretation as a nonlinear sigma model on a complete intersection Calabi-Yau manifold. The complete intersection Calabi-Yau manifold should be equivalent to the Calabi-Yau supermanifold prescribed by Sethi's proposal.Comment: 24 pages, uses JHEP3.cls; v2: minor corrections, references adde

Single-cell analysis of CHO cells reveals clonal heterogeneity in hyperosmolality-induced stress response

Hyperosmolality can occur during industrial fed-batch cultivation processes of Chinese hamster ovary (CHO) cells as highly concentrated feed and base solutions are added to replenish nutrients and regulate pH values. Some effects of hyperosmolality, such as increased cell size and growth inhibition, have been elucidated by previous research, but the impact of hyperosmolality and the specific effects of the added osmotic-active reagents have rarely been disentangled. In this study, CHO cells were exposed to four osmotic conditions between 300 mOsm/kg (physiologic condition) and 530 mOsm/kg (extreme hyperosmolality) caused by the addition of either high-glucose-supplemented industrial feed or mannitol as an osmotic control. We present novel single-cell cultivation data revealing heterogeneity in mass gain and cell division in response to these treatments. Exposure to extreme mannitol-induced hyperosmolality and to high-glucose-oversupplemented feed causes cell cycle termination, mtDNA damage, and mitochondrial membrane depolarization, which hints at the onset of premature stress-induced senescence. Thus, this study shows that both mannitol-induced hyperosmolality (530 mOsm/kg) and glucose overfeeding induce severe negative effects on cell growth and mitochondrial activity; therefore, they need to be considered during process development for commercial production

Binary planetesimals and their role in planet formation

One of the main evolutionary stages of planet formation is the dynamical evolution of planetesimal disks. These disks are thought to evolve through gravitational encounters and physical collisions between single planetesimals. In recent years, many binary planetesimals have been observed in the Solar system, indicating that the binarity of planetesimals is high. However, current studies of planetesimal disks formation and evolution do not account for the role of binaries. Here we point out that gravitational encounters of binary planetesimals can have an important role in the evolution of planetesimal disks. Binary planetesimals catalyze close encounters between planetesimals, and can strongly enhance their collision rate. Binaries may also serve as additional heating source of the planetesimal disk, through the exchange of the binaries gravitational potential energy into the kinetic energy of planetesimals in the disk.Comment: Accepted to ApJ

The angular momentum of two collided rarefied preplanetesimals and the formation of binaries

This paper studies the mean angular momentum associated with the collision of two celestial objects in the earliest stages of planet formation. Of primary concern is the scenario of two rarefied preplanetesimals (RPPs) in circular heliocentric orbits. The theoretical results are used to develop models of binary or multiple system formation from RPPs, and explain the observation that a greater fraction of binaries originated farther from the Sun. At the stage of RPPs, small-body satellites can form in two ways: a merger between RPPs can have two centers of contraction, or the formation of satellites from a disc around the primary or the secondary. Formation of the disc can be caused by that the angular momentum of the RPP formed by the merger is greater than the critical angular momentum for a solid body. One or several satellites of the primary (moving mainly in low-eccentricity orbits) can be formed from this disc at any separation less than the Hill radius. The first scenario can explain a system such as 2001 QW322 where the two components have similar masses but are separated by a great distance. In general, any values for the eccentricity and inclination of the mutual orbit are possible. Among discovered binaries, the observed angular momenta are smaller than the typical angular momenta expected for identical RPPs having the same total mass as the discovered binary and encountering each other in circular heliocentric orbits. This suggests that the population of RPPs underwent some contraction before mergers became common.Comment: 12 pages, Monthly Notices of Royal Astron. Society, in pres

Characterization of the human AGE1.HN cell line: a systems biology approach

Scholz S, Luebbecke M, Rath A, et al. Characterization of the human AGE1.HN cell line: a systems biology approach. BMC Proceedings. 2011;5(8)

Relative Configuration of Micrograms of Natural Compounds Using Proton Residual Chemical Shift Anisotropy

[Abstract] 3D molecular structure determination is a challenge for organic compounds or natural products available in minute amounts. Proton/proton and proton/carbon correlations yield the constitution. J couplings and NOEs oftentimes supported by one-bond 1H,13C residual dipolar couplings (RDCs) or by 13C residual chemical shift anisotropies (RCSAs) provide the relative configuration. However, these RDCs or carbon RCSAs rely on 1% natural abundance of 13C preventing their use for compounds available only in quantities of a few 10’s of µgs. By contrast, 1H RCSAs provide similar information on spatial orientation of structural moieties within a molecule, while using the abundant 1H spin. Herein, 1H RCSAs are accurately measured using constrained aligning gels or liquid crystals and applied to the 3D structural determination of molecules with varying complexities. Even more, deuterated alignment media allow the elucidation of the relative configuration of around 35 µg of a briarane compound isolated from Briareum asbestinum.This work was supported by the Max Planck Society and grew out of a collaboration in the context of the Forschergruppe (FOR 934) continued now by the DFG (Gr1211/19–1 and Re1007/9–1)/CAPES 418729698 project. N.N. gratefully acknowledges the financial support by SERB, New Delhi for ECR Grant with File No.: ECR/2017/001811. This work was also funded by grants RTI2018-093634-B-C22 from the State Agency for Research (AEI) of Spain, both co-funded by the FEDER Programme from the European Union, BLUEBIOLAB (0474_BLUEBIOLAB_1_E), Programme INTERREG V A of Spain-Portugal (POCTEP) and GRC2018/039 and Agrupación Estratégica CICA-INIBIC ED431E 2018/03 from Xunta de Galicia. C.J., J.R., and D.P.P. acknowledge CESGA for the computational support. J.C.F. acknowledges predoctoral research stay grant Inditex-UDC. D.P.P. received a fellowship from the program National Council of Science and Technology (CONACYT) of Mexico and the Secretariat of Research, Innovation and Higher Education (SIIES) of Yucatan (Mexico). We also thank Dr. G. Jithender Reddy for one isotropic measurement. We also thank Dr. Christian Schmidt for his cooperation in the manufacturing of micro stretching device. ANV thanks CNPq for a research fellowship and financial support M(426216/2018–0)German Research Foundation; Gr1211/19–1German Research Foundation; Re1007/9–1Brasil. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); 418729698República de la India. Science and Engineering Research Board; ECR/2017/001811Xunta de Galicia; 0474_BLUEBIOLAB_1_EXunta de Galicia; GRC2018/039Xunta de Galicia; ED431E 2018/03Brasil. Conselho Nacional de Desenvolvimento Científico e Tecnológico; M(426216/2018–0